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Drug Delivery Plasmid

Targeted for Her-2 Positive Breast Cancer
Course: Biomolecular Engineering
Lab: Dr. Chemeng Tan, UC Davis

For a course project, we created a plan to modify surface proteins to enhance the migration and binding to breast cancer. Normally, multiple receptor approaches are limited by pharmacokinetics, but our hypothesis is that E. Coli can take advantage of multiple receptors for targeting. The design of the plasmid that was to be transformed into E. Coli can be seen below. 

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Our project was invited to come to fruition in Dr. Chemeng Tan's Lab after the semester ended. Our group was able to order the a parts and begin assembly of our design through Gibson Assembly and golden gate PCR.  The project was continued by PHD's in the lab following our graduation.

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Plasmid Design

Using fusion protein method:
RGD with OmpA
ZHER2:342 with AIDA-I

Skills and Tools

Technical skills learned or used from this experience 

Software

Benchmark

Cell Skills

PCR

Gel Electrophoresis

Media preparation

Topics

Cellular Systems

Research Experience

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