Drug Delivery Plasmid
Targeted for Her-2 Positive Breast Cancer
Course: Biomolecular Engineering
Lab: Dr. Chemeng Tan, UC Davis
For a course project, we created a plan to modify surface proteins to enhance the migration and binding to breast cancer. Normally, multiple receptor approaches are limited by pharmacokinetics, but our hypothesis is that E. Coli can take advantage of multiple receptors for targeting. The design of the plasmid that was to be transformed into E. Coli can be seen below.
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Our project was invited to come to fruition in Dr. Chemeng Tan's Lab after the semester ended. Our group was able to order the a parts and begin assembly of our design through Gibson Assembly and golden gate PCR. The project was continued by PHD's in the lab following our graduation.
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Plasmid Design

Using fusion protein method:
RGD with OmpA
ZHER2:342 with AIDA-I
Skills and Tools
Technical skills learned or used from this experience
Software
Benchmark
Cell Skills
PCR
Gel Electrophoresis
Media preparation
Topics
Cellular Systems
Research Experience